dapi nuclear staining (Beyotime)
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dapi nuclear staining
Dapi Nuclear Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+nuclear+stain/DAPI/pm41981622-177-9-12
Average 99 stars, based on 32109 article reviews
Dapi Nuclear Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+nuclear+stain/DAPI/pm41981622-177-9-12
Average 99 stars, based on 32109 article reviews
dapi nuclear staining - by Bioz Stars,
2026-09
99/100 stars
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Staining:Article Title: Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Article Snippet: Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).. Rupture of lysosome is a major cellular stress condition leading to cell death in PD.. We have previously shown that environmental oxidative toxins could impair autophagic flux and lysosomal functions in PD. Article Title: A long non-coding RNA essential for early embryonic development improves somatic cell nuclear transfer somatic cell nuclear transfer efficiency in goats Article Snippet: Afterward, the samples were rinsed three times for 5 min each with PBS-PVA, incubated with goat anti-rabbit IgG H&L (Alexa Fluor® 488) (1:500; ab150077, Abcam) or goat anti-mouse IgG (H+L) highly crossadsorbed secondary antibody, Alexa FluorTM Plus 555 (1:500; A32727, Invitrogen) for 2 h at room temperature in the dark and rinsed with PBS-PVA. .. DAPI (1:500; C1005, Beyotime) Article Title: Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis-fibroblast-like synoviocytes by inhibiting the NF-κB and MAPK signaling pathways Article Snippet: PIC (>98% pure) was purchased from Nantong FeiYu Biological Technology Co., Ltd. .. Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Molecular Technologies, Inc. Rat TNF-α (cat. no. RTA00), IL-1β (cat. no. PRLB00), PGE2(KGE004B) and IL-6 (cat. no. R6000B) ELISA kits were purchased from R&D Systems, Inc. Article Title: Down‐Regulation of SOX2 Expression by pH‐Responsive Micelles Inhibits the Formation of Invadopodia in Prostate Cancer and Enhances the Effect of Chemotherapy Article Snippet: The followingmaterialswere used: Chemical reagents: Pronethalol (MW = 259.34, analytical grade, ≥99%, Cat. No. HY-B1238; MedChemExpress); DSPE-PEGCOOH (MW= 2780.38, analytical grade,≥99%, CAS 1403744-37-5; MACKLIN); Doxorubicin (DOX, MW = 579.98, analytical grade, ≥99%, CAS 25316-40-9; MACKLIN); Dicyclohexylcarbodiimide (DCC, MW = 206.33, analytical grade, ≥99%, Cat. No. HYY1092; MedChemExpress);4-Dimethylaminopyridine (DMAP, MW= 122.17, Analytical grade,≥99%, CAS 1122-58-3; MACKLIN); Dichloromethane (DCM, MW = 84.93, analytical grade, ≥99%, CAS 75-09-2; MACKLIN); Phosphate-buffered saline (PBS, pH 7.4, Cat. No. C0221B; Beyotime). .. RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS); CCK-8 cell viability assay kit (C0038; Beyotime); Article Title: Molecular mechanisms of Batai virus entry into mouse neuroblastoma N2a cells via Clathrin-Mediated Endocytosis. Article Snippet: Batai virus (BATV), an arbovirus belonging to the family Peribunyaviridae, has garnered increasing attention due to its broad host range, expanding geographical distribution, and capacity to naturally infect the mammalian central nervous system with the potential for evolution into more virulent strains.. As an enveloped virus, BATV enters host cells via endocytosis, with clathrin-mediated endocytosis (CME) representing the most extensively characterized entry mechanism.. This study systematically investigated the molecular mechanisms underlying BATV entry into mouse neuroblastoma N2a cells via CME using pharmacological inhibitors, dominant-negative mutants, and siRNA interference approaches. Article Title: Mechanical stimulation of extracellular vesicles secreted by bone marrow mesenchymal stem cells promotes osteoblast proliferation and differentiation by activating the Wnt/β - catenin signaling pathway. Article Snippet: Introduction: Osteoporosis is characterized by decreased bone mass, microstructural deterioration of bone tissue, and increased bone fragility.. Bone marrow mesenchymal stem cells (BMSCs) are essential for bone growth and repair.. Exosomes, which are key mediators of intercellular communication, participate in various biological processes. Article Title: Simvastatin accelerates hematoma resolution after intracerebral hemorrhage in a PPARγ-dependent manner. Article Snippet: Accepted Manuscript Simvastatin accelerates hematoma resolution after intracerebral hemorrhage in a PPARγ-dependent manner Yuelong Wang, Qianwei Chen, Qiang Tan, Zhou Feng, Zhenlin He, Jun Tang, Hua Feng, Gang Zhu, Zhi Chen PII: S0028-3908(17)30488-4 DOI: 10.1016/j.neuropharm.2017.10.021 Reference: NP 6906 To appear in: Neuropharmacology Received Date: 10 July 2017 Revised Date: 19 September 2017 Accepted Date: 16 October 2017 Please cite this article as: Wang, Y., Chen, Q., Tan, Q., Feng, Z., He, Z., Tang, J., Feng, H., Zhu, G., Chen, Z., Simvastatin accelerates hematoma resolution after intracerebral hemorrhage in a PPARγdependent manner, Neuropharmacology (2017), doi: 10.1016/j.neuropharm.2017.10.021.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript. End Labeling:Article Title: Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Article Snippet: Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).. Rupture of lysosome is a major cellular stress condition leading to cell death in PD.. We have previously shown that environmental oxidative toxins could impair autophagic flux and lysosomal functions in PD. TUNEL Assay:Article Title: Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Article Snippet: Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).. Rupture of lysosome is a major cellular stress condition leading to cell death in PD.. We have previously shown that environmental oxidative toxins could impair autophagic flux and lysosomal functions in PD. Apoptosis Assay:Article Title: Poloxamer 188 rescues MPTP-induced lysosomal membrane integrity impairment in cellular and mouse models of Parkinson's disease. Article Snippet: Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc).. Rupture of lysosome is a major cellular stress condition leading to cell death in PD.. We have previously shown that environmental oxidative toxins could impair autophagic flux and lysosomal functions in PD. CCK-8 Assay:Article Title: Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis-fibroblast-like synoviocytes by inhibiting the NF-κB and MAPK signaling pathways Article Snippet: PIC (>98% pure) was purchased from Nantong FeiYu Biological Technology Co., Ltd. .. Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Molecular Technologies, Inc. Rat TNF-α (cat. no. RTA00), IL-1β (cat. no. PRLB00), PGE2(KGE004B) and IL-6 (cat. no. R6000B) ELISA kits were purchased from R&D Systems, Inc. Article Title: Down‐Regulation of SOX2 Expression by pH‐Responsive Micelles Inhibits the Formation of Invadopodia in Prostate Cancer and Enhances the Effect of Chemotherapy Article Snippet: The followingmaterialswere used: Chemical reagents: Pronethalol (MW = 259.34, analytical grade, ≥99%, Cat. No. HY-B1238; MedChemExpress); DSPE-PEGCOOH (MW= 2780.38, analytical grade,≥99%, CAS 1403744-37-5; MACKLIN); Doxorubicin (DOX, MW = 579.98, analytical grade, ≥99%, CAS 25316-40-9; MACKLIN); Dicyclohexylcarbodiimide (DCC, MW = 206.33, analytical grade, ≥99%, Cat. No. HYY1092; MedChemExpress);4-Dimethylaminopyridine (DMAP, MW= 122.17, Analytical grade,≥99%, CAS 1122-58-3; MACKLIN); Dichloromethane (DCM, MW = 84.93, analytical grade, ≥99%, CAS 75-09-2; MACKLIN); Phosphate-buffered saline (PBS, pH 7.4, Cat. No. C0221B; Beyotime). .. RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS); CCK-8 cell viability assay kit (C0038; Beyotime); Enzyme-linked Immunosorbent Assay:Article Title: Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis-fibroblast-like synoviocytes by inhibiting the NF-κB and MAPK signaling pathways Article Snippet: PIC (>98% pure) was purchased from Nantong FeiYu Biological Technology Co., Ltd. .. Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Molecular Technologies, Inc. Rat TNF-α (cat. no. RTA00), IL-1β (cat. no. PRLB00), PGE2(KGE004B) and IL-6 (cat. no. R6000B) ELISA kits were purchased from R&D Systems, Inc. Viability Assay:Article Title: Down‐Regulation of SOX2 Expression by pH‐Responsive Micelles Inhibits the Formation of Invadopodia in Prostate Cancer and Enhances the Effect of Chemotherapy Article Snippet: The followingmaterialswere used: Chemical reagents: Pronethalol (MW = 259.34, analytical grade, ≥99%, Cat. No. HY-B1238; MedChemExpress); DSPE-PEGCOOH (MW= 2780.38, analytical grade,≥99%, CAS 1403744-37-5; MACKLIN); Doxorubicin (DOX, MW = 579.98, analytical grade, ≥99%, CAS 25316-40-9; MACKLIN); Dicyclohexylcarbodiimide (DCC, MW = 206.33, analytical grade, ≥99%, Cat. No. HYY1092; MedChemExpress);4-Dimethylaminopyridine (DMAP, MW= 122.17, Analytical grade,≥99%, CAS 1122-58-3; MACKLIN); Dichloromethane (DCM, MW = 84.93, analytical grade, ≥99%, CAS 75-09-2; MACKLIN); Phosphate-buffered saline (PBS, pH 7.4, Cat. No. C0221B; Beyotime). .. RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS); CCK-8 cell viability assay kit (C0038; Beyotime); Membrane:Article Title: Down‐Regulation of SOX2 Expression by pH‐Responsive Micelles Inhibits the Formation of Invadopodia in Prostate Cancer and Enhances the Effect of Chemotherapy Article Snippet: The followingmaterialswere used: Chemical reagents: Pronethalol (MW = 259.34, analytical grade, ≥99%, Cat. No. HY-B1238; MedChemExpress); DSPE-PEGCOOH (MW= 2780.38, analytical grade,≥99%, CAS 1403744-37-5; MACKLIN); Doxorubicin (DOX, MW = 579.98, analytical grade, ≥99%, CAS 25316-40-9; MACKLIN); Dicyclohexylcarbodiimide (DCC, MW = 206.33, analytical grade, ≥99%, Cat. No. HYY1092; MedChemExpress);4-Dimethylaminopyridine (DMAP, MW= 122.17, Analytical grade,≥99%, CAS 1122-58-3; MACKLIN); Dichloromethane (DCM, MW = 84.93, analytical grade, ≥99%, CAS 75-09-2; MACKLIN); Phosphate-buffered saline (PBS, pH 7.4, Cat. No. C0221B; Beyotime). .. RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS); CCK-8 cell viability assay kit (C0038; Beyotime); Transfection:Article Title: Molecular mechanisms of Batai virus entry into mouse neuroblastoma N2a cells via Clathrin-Mediated Endocytosis. Article Snippet: Batai virus (BATV), an arbovirus belonging to the family Peribunyaviridae, has garnered increasing attention due to its broad host range, expanding geographical distribution, and capacity to naturally infect the mammalian central nervous system with the potential for evolution into more virulent strains.. As an enveloped virus, BATV enters host cells via endocytosis, with clathrin-mediated endocytosis (CME) representing the most extensively characterized entry mechanism.. This study systematically investigated the molecular mechanisms underlying BATV entry into mouse neuroblastoma N2a cells via CME using pharmacological inhibitors, dominant-negative mutants, and siRNA interference approaches. Immunofluorescence:Article Title: Molecular mechanisms of Batai virus entry into mouse neuroblastoma N2a cells via Clathrin-Mediated Endocytosis. Article Snippet: Batai virus (BATV), an arbovirus belonging to the family Peribunyaviridae, has garnered increasing attention due to its broad host range, expanding geographical distribution, and capacity to naturally infect the mammalian central nervous system with the potential for evolution into more virulent strains.. As an enveloped virus, BATV enters host cells via endocytosis, with clathrin-mediated endocytosis (CME) representing the most extensively characterized entry mechanism.. This study systematically investigated the molecular mechanisms underlying BATV entry into mouse neuroblastoma N2a cells via CME using pharmacological inhibitors, dominant-negative mutants, and siRNA interference approaches. Blocking Assay:Article Title: Molecular mechanisms of Batai virus entry into mouse neuroblastoma N2a cells via Clathrin-Mediated Endocytosis. Article Snippet: Batai virus (BATV), an arbovirus belonging to the family Peribunyaviridae, has garnered increasing attention due to its broad host range, expanding geographical distribution, and capacity to naturally infect the mammalian central nervous system with the potential for evolution into more virulent strains.. As an enveloped virus, BATV enters host cells via endocytosis, with clathrin-mediated endocytosis (CME) representing the most extensively characterized entry mechanism.. This study systematically investigated the molecular mechanisms underlying BATV entry into mouse neuroblastoma N2a cells via CME using pharmacological inhibitors, dominant-negative mutants, and siRNA interference approaches. other:Article Title: Electroacupuncture regulates microglia activation through the STING/NF- κ B pathway to reduce pain in bone cancer pain model Article Snippet: The brain slices were then rinsed with the Incubation:Article Title: Mechanical stimulation of extracellular vesicles secreted by bone marrow mesenchymal stem cells promotes osteoblast proliferation and differentiation by activating the Wnt/β - catenin signaling pathway. Article Snippet: Introduction: Osteoporosis is characterized by decreased bone mass, microstructural deterioration of bone tissue, and increased bone fragility.. Bone marrow mesenchymal stem cells (BMSCs) are essential for bone growth and repair.. Exosomes, which are key mediators of intercellular communication, participate in various biological processes. |
